Antimicrobial Property of Silver Nanoparticles: Effects of Concentration and Temperature on Bacterial Isolates
نویسندگان
چکیده
Importance of hospital environment in patient-care has been recognized widely infection prevention and control. Inappropriate antibiotic use led to emergence resistant strains that are difficult treat with the available antibiotics. Progress nanotechnology enhancement nanoparticles physicochemical characteristics functionality overcomes constraints common antimicrobials. Aim was investigate effective antimicrobial role Silver nanoparticle (Ag-NPs) against clinically important bacterial observe effects varying storage temperatures on Ag-NPs activity. Different concentrations were tested Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa Acinetobacter baumannii using diffusion method. Zone inhibition (ZOI) for each organism directly proportional concentration used. Mean ZOI values at different significantly all organisms p-value <0.001 E. S. P. 0.004 A. baumannii. Variation temperature hardly showed any property Ag-NPs. Scanning electron microscopy (SEM) morphological size variations exposed cells when compared control strains, especially coli aeruginosa. Damaged cell membrane areas can be clearly distinguished thus suggesting disruption. These finding help design a larger study where used various medical instruments which usually kept room temperatures. Also, outcomes this may designing proper implants, prosthesis equipment coated minimum might considered safe applications.
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ژورنال
عنوان ژورنال: Journal of Pure and Applied Microbiology
سال: 2023
ISSN: ['2581-690X', '0973-7510']
DOI: https://doi.org/10.22207/jpam.17.2.42